Catalog Number - 62308-05P-2

Hyper Similars C8™ Octyl is a traditional reversed-phase HPLC stationary phase developed for laboratories seeking a high-quality alternative to established C8 methods. As part of the Cogent™ Similars product line, it uses high-purity Type-B spherical silica with a bonded octyl (C8) ligand and endcapping to provide reliable chromatographic performance and excellent method reproducibility. Its surface characteristics are designed to closely match those of legacy Hypersil® and Spherisorb® C8 columns, making it particularly useful for method transfer and replacement applications.

62308-05P-2 is a 50 mm x 2.1 mm ID narrow-bore analytical column packed with 3 µm, 150Å Hyper Similars C8 particles. The short 50 mm length provides rapid analyses and reduced solvent consumption, while the 2.1 mm ID format is optimized for low-flow applications and LC-MS methods. The 3 µm particle size delivers higher efficiency and sharper peaks than comparable 5 µm columns, although higher operating pressures should be expected. Optimal flow rates are typically approximately 0.2–0.3 mL/min, and the HPLC or LC-MS system should be capable of delivering precise, stable low-flow performance with appropriately low-volume flow cells and connections.

This USP L7 column is an excellent alternative for methods developed on Thermo Fisher Hypersil™ C8, Spherisorb®, and similar octyl phases. The C8 chemistry is well suited for hydrophobic and neutral compounds analyzed under reversed-phase conditions and is particularly useful when less retention is desired than a C18 column provides. The narrow-bore format has become a preferred choice for LC-MS applications due to reduced solvent consumption, enhanced MS compatibility, and improved sensitivity.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L7 methods

• Comparable selectivity to Hypersil® and Spherisorb® C8 columns

• Narrow-bore format ideal for LC-MS applications

• Fast analysis with 50 mm column length

• Higher efficiency with 3 µm particles

• Reduced solvent consumption

• Less retention than C18 columns

Typical Applications

• USP L7 methods

• LC-MS analysis

• Reversed-phase HPLC

• Fast analytical methods

• Pharmaceutical analysis

• Quality control testing

• Long-chain fatty acid analysis

• High-throughput laboratories

Specifications

• Product Type: HPLC Column

• Stationary Phase: Hyper Similars C8 Octyl

• USP Classification: L7

• Particle Size: 3 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C8 (Octyl)

• Endcapped: Yes

• Pore Size: 150Å

• Column Dimensions: 2.1 mm ID x 50 mm

• Length: 50 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Narrow-bore format is ideal for LC-MS methods

• Requires an HPLC or LC-MS system capable of precise low-flow operation

• 3 µm particles provide higher efficiency and sharper peaks

• Higher backpressure than comparable 5 µm columns

• Reduced solvent consumption compared to 4.6 mm ID columns

• Excellent alternative for many USP L7 methods

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